Double Negative T Cells Downregulate Intrahepatic Inflammation and Ameliorate Hepatic Ischemia and Reperfusion Injury via CD39-adenosinergic axis [RNA-seq]

Converted double negative T cells (cDNT) highly express CD39 and hydrolyze ATP into adenosine. ATP/adenosine derived purinergic signaling plays a critical role in immune regulation. However, the role and mechanisms of ATP/adenosine in cDNT is unclear. Overall design: To investigate the role of purinergic signaling on cDNT, we treated DNT with or without ATPgs (a non-hydrolysable ATP analogue), adenosine, adenosine receptor A2a inhibitor (A2aRi) and performed mRNA transcriptome sequencing.