Effects of rosiglitazone on human omental adipose tissue gene expression

The cellular mechanisms by which PPARγ improves metabolism in mature human adipocytes and potential depot differences in its actions are poorly understood. To assess the global effects of PPARγ activation on human omental gene expression, we conducted microarray analysis of control vs. rosiglitazone-treated omental tissues from human subjects. Adipose tissues fragemnts from 2 independent donors were treated with rosigltiazone (μM) for 7 days. Total RNA was extracted and used for micorray analysis using Affymatrix. Technical duplicates of the first subject were averaged with the values from the second subject prior to statistical analysis.