Single-cell and bulk RNA sequencing of mouse atherosclerosis disease stage course [TRAP-seq]

Atherosclerotic coronary artery disease (CAD) development encompasses changes in immune cell activation across different tissues, including vascular and metabolic tissues. However, the specific disease-associated transcriptional changes occurring within each of the tissue cell types remain incompletely characterized. Here, we combine an atherosclerosis mouse model with a mouse strain designed for translating ribosome affinity purification followed by RNA sequencing (TRAP-Seq). The mice express a fusion protein of EGFP and ribosomal protein L10a (EGFP-L10a) under the control of the macrophage-specific Csf1r (Cfms) promoter. Using EGFP as an affinity tag, this enables the enrichment of macrophage-specific ribosome-associated RNA from whole tissue lysates. Here, we profile the macrophage-enriched RNA fraction from aorta, liver and adipose tissue.动脉粥样硬化性冠状动脉疾病（CAD）的发生发展涉及包括血管与代谢组织在内的多种组织中免疫细胞激活状态的改变。然而，各组织细胞类型内与疾病特异性相关的转录组变化仍未得到充分表征。本研究将动脉粥样硬化小鼠模型与适配翻译核糖体亲和纯化联合RNA测序（TRAP-Seq）的小鼠品系相结合。该小鼠在巨噬细胞特异性Csf1r（Cfms）启动子的调控下，表达增强绿色荧光蛋白与核糖体蛋白L10a的融合蛋白（EGFP-L10a）。以EGFP作为亲和标签，该系统可从全组织裂解液中富集巨噬细胞特异性的核糖体结合RNA。本研究对主动脉、肝脏及脂肪组织中的巨噬细胞富集RNA组分开展了转录组谱分析。