Hormonal Regulation of MicroRNA Expression in Rat Adrenal Gland

To determine miRNA profiles in rat adrenals from animals treated with vehicle, ACTH, 17a-ethinyl estradiol (17a-E2) or dexamethasone (DEX). miRNA expression profiling was performed using Affymetrix® GeneChip® miRNA 2.0 Arrays. Together with qRT-PCR, several adrenal miRNAs have been identified, whose expression is subject to regulation by one or more than one hormone.本研究旨在明确经赋形剂、促肾上腺皮质激素（adrenocorticotropic hormone, ACTH）、17α-乙炔雌二醇（17α-E2）或地塞米松（DEX）处理的大鼠肾上腺组织中的miRNA（microRNA）表达谱。本研究采用Affymetrix® GeneChip® miRNA 2.0 阵列芯片进行miRNA表达谱分析，并结合实时定量逆转录聚合酶链反应（quantitative real-time polymerase chain reaction, qRT-PCR），最终鉴定出多种肾上腺miRNA，其表达可受一种或多种激素的调控。