A 21-marker 18-color flow cytometry panel for in-depth phenotyping of human peripheral monocytes.

This 18-marker, 21-color panel has been designed to delineate and characterize four subsets of monocytes (classical, intermediate, SLAN- non-classical and SLAN+ non-classical) from peripheral human blood. Conclusion: We successfully optimized an 18-marker, 21-color monocyte-centric human immunophenotyping panel. Cytek CQ calibration beads were used for instrumental QC, performed at every run. The QC software adjusts laser delays, area scaling factors and gains settings to account for instrument variability over time.