RNA sequencing of patient derived xenograft sample AML-346, AML-356, AML-388 and AML-661 using prime-seq

We generate CRISPR/Cas9 patient derived xenograft (PDX) models of acute myeloid leukemia. Mimicking anti-cancer therapy in patients, PDX cells with gene knockouts were re-transplanted into mice. In fluorochromes guided, competitive in vivo trials, a subset of PDX models showed a clear growth disadvantage upon knockout, indicating essential functions for WT1 and DNMT3A. A mixture of WT1 KOs, DNMT3A KOs and control PDX cells were isolated out of the murine bone marrow after the mice reached advanced leukemia stage and sorted for single population.The mRNA expression pattern was analyzed. ---------------------------- Authors state "Due to German law, we are not allowed to publish raw sequencing data."