APP/PS1 mice characterization and time progression

RNA samples from the cerebral cortex of APP/PS1 and WT mouse littermates aged 3, 6 and 12 months were analyzed using the Affymetrix Genechip Mouse Gene 1.1 ST Array. The APP-PS1 transgenic mouse express the human mutated forms APPswe and PS1dE9. This is a good model of familial Alzheimer Disease because it reproduces several features of the disease as beta-amyloid deposits throughout the brain and exhibit memory impairment by the end of the sixth month and is a simple model to study the molecular pathways. The aim of this study is to identify dysregulation of inflammation pathways in order to understand shifts of inflammation responses with disease progression.本研究采用Affymetrix GeneChip 小鼠基因1.1 ST 阵列，对3、6及12月龄的APP/PS1双转基因小鼠与同窝野生型（Wild Type, WT）小鼠的大脑皮层RNA样本进行了分析。该APP/PS1转基因小鼠可表达人源突变形式的APPswe与PS1dE9。作为家族性阿尔茨海默病（familial Alzheimer Disease）的理想动物模型，其可重现该病的多项核心病理特征：全脑广泛分布的β-淀粉样蛋白（β-amyloid）沉积，并于6月龄末期出现记忆障碍，是用于探究炎症相关分子通路的简便模型。本研究旨在鉴定炎症通路的表达失调情况，以阐明疾病进展过程中炎症应答的动态变化。