RNA-seq of developing grain tissues (12 days post-anthesis) of red (R-D1b) and white (R-D1a) near-isogenic lines of wheat cv. Holdfast.

Proanthocyanidin (PA) is a polymeric flavonoid found in the seed coat of many plant species, including wheat, and is responsible for the red grain colour of most wheat cultivars. White wheat cultivars that lack PA have null mutations in all three homoeologues of the gene encoding the R myb transcription factor. Previous work has shown that R control several genes in the PA biosynthetic pathway. The aim of this experiment was to identify other components of the PA pathway of wheat controlled by R through comparison of transcript levels in tissues of developing grain of red and white near-isogenic lines of the cultivar Holdfast. Note: RNA-seq reads from the Red samples in this experiment have previously been submitted to ENA under the accession number E-MTAB-3103.