Plant endosphere metagenome (16S/ITS/nifH amplicons): leaf and root endophytic microbiomes of 10 Rhododendron species from the southeastern Tibetan Plateau

This project investigates plant endosphere metagenomes of leaf and root tissues from 10 Rhododendron species collected along an elevational gradient in Linzhi, Xizang (southeastern Tibetan Plateau, China). Three individual plants (>=50 m apart) were sampled per species. Leaves were collected from four cardinal directions and pooled, and fine roots (<1 mm) were aseptically collected, yielding 60 tissue samples in total. Amplicon sequencing was performed on an Illumina MiSeq platform (paired-end), targeting the bacterial 16S rRNA gene, the fungal ITS1 region, and the nitrogenase nifH gene. Raw demultiplexed FASTQ files are provided for downstream analyses of tissue- and host-associated microbiome variation.