The effects of differential gut microbiota on hepatic gene expression. The effects of differential gut microbiota on hepatic gene expression

Our preliminary data suggest that differential gut microbiota modulates acetaminophen-induced hepatotoxicity (APAP toxicity) in mice model. The goal of our study is to determine whether commensal gut microbiota modulates the hepatic gene expressions potentially responsible for modulating APAP toxicity. Overall design: Method: C57BL/6 male mice from two different vendors [Jackson (JAX) and Taconic (TAC)] were sacrificed and liver tissue collected. Cecum materials from JAX or TAC mice were inoculated to germ-free (GF) mice. After 4 weeks of conventionalization, mice were overnight fasted (16 h) and sacrificed for liver collection. Examination of 5 groups; JAX, TAC, GF, GF_JAX and GF_TAC