This experiment was performed to identify somatic hypermutations in germinal center B cells of a mouse expressing the inferred germline of IOMA BCR after and a 5 step sequential immunization regimen.
Oxford Nanopore ultra-long sequencing with adaptive sampling is used to sequence the immunoglobulin heavy chain (IGH) locus. The reads were used to phase and generate single-contig assemblies of the l
The processes of somatic hypermutation (SHM) and class switch recombination introduced by activation-induced cytosine deaminase (AICDA) at the Immunoglobulin (Ig) loci are key steps for creating a poo
