Mechanisms Governing Target Search and Binding Dynamics of Hypoxia-Inducible Factors [ChIP-seq]

We performed ChIP-seq to compare genome-wide HIF-1b and HIF-2a occupancy in wild type 786-O cells to 786-O cells with either HIF-1b or HIF-2a endogenously tagged with a V5-Halo moiety. We performed ChIP-seq in WT 786-O cells and in 786-O cells with either HIF-1b or HIF-2a endogenously tagged with a V5-Halo moiety using no antibody (input samples) or anti-HIF-1b, anti-HIF-2a and anti-V5 antibodies (3 cell types, 3 antibodies plus inputs for a total of 12 libraries).