RNA-sequencing in mESCs with overexpression of mCherry-tagged DUX and DUX domain derivatives

To investigate the requirement of DUX domains for target gene expression, we created clonal stable cell lines in Dux-/- mouse embryonic stem cells and expressed constructs for 12 hours with 0.25 ug/mL doxycycline We then performed gene expression profiling analysis using data obtained from RNA-seq of 11 different cell lines at one time point Overall design: Comparative gene expression profiling analysis of RNA-seq data for mCherry-tagged DUX and DUX domain derivative constructs in mESCs