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HeLa standard shotgun DDA analysis using a two-meter C18 monolithic silica column

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https://www.omicsdi.org/dataset/jpost/PXD005159
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Human HeLa proteome analysis by a nanoLC-MS/MS system using a two-meter C18 monolithic silica column under shallow gradient conditions without pre-fractionation was performed in this project. A hybrid quadrupole-orbitrap mass spectrometer (Thermo Q-Exactive) was used to analyze a tryptic digest of HeLa cells through this study. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.
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2016-10-17
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