Lipidomic dataset of MDA-MB-231 human breast cancer cells treated with diacylglycerol acyltransferase (DGAT) inhibitors and docosahexaenoic acid (DHA)

Lipid droplets (LDs) are dynamic fat storage organelles involved in fatty acid metabolism. By storing polyunsaturated fatty acids (PUFAs) in the form of neutral lipids, LDs can either mitigate or exacerbate lipotoxic damage. However, their role in regulating cellular fatty acid distribution, membrane unsaturation, and ferroptosis susceptibility remains poorly understood. To explore how LD turnover modulates membrane lipid composition and ferroptosis sensitivity in cancer cells exposed to exogenous PUFAs, MDA-MB-231 human triple-negative breast cancer cells were supplemented with non-lethal concentrations of exogenous docosahexaenoic acid (DHA) and treated with inhibitors of diacylglycerol acyltransferases (DGAT) 1 and 2. Untargeted lipidomic analysis revealed substantial lipidome remodeling with 302 significantly altered lipids in response to DGAT inhibition, DHA supplementation, or combined treatment.
, Sample Type:
Total cell lysates of MDA-MB-231 cells.
Cell Culture and Sample Preparation:
MDA-MB-231 cells were seeded in 6-well plates at 3 × 10^5 cells/well and left to attach for 24 h. After 24 h, the cells were treated with DGAT inhibitors and 25 μM DHA in RPMI-1640 media supplemented with 10% FBS. After 1, 4, and 24 h, cell lysates were collected. Total protein content was determined in each sample using the Pierce 660 nm Protein Assay Kit following the manufacturer’s instructions and used to normalize lipid signals.
Lipid Extraction for Mass Spectrometry:
Lipids were extracted using the LIMeX LC–MS workflow, including biphasic solvent system consisting of cold methanol, methyl tert-butyl ether, and 10% methanol. An aliquot of the upper organic phase was collected, evaporated, and resuspended in methanol containing the internal standard [12-[(cyclohexylamino)carbonyl]amino]-dodecanoic acid (CUDA).
LC-MS Data Acquisition and Lipid Annotation:
For untargeted lipid..., # Lipidomic dataset of MDA-MB-231 human breast cancer cells treated with diacylglycerol acyltransferase (DGAT) inhibitors and docosahexaenoic acid (DHA)

Dataset DOI: [10.5061/dryad.2bvq83c4h](https://doi.org/10.5061/dryad.2bvq83c4h)

## Description of the data and file structure

Human MDA-MB-231 cancer cells were treated with a mixture of DGAT1 and DGAT2 inhibitors, supplemented with exogenous DHA, harvested at three time points, and analyzed by LC/MS using the LIMex platform. Untargeted lipidomic analysis revealed substantial lipidome remodeling with 302 significantly altered lipids in response to DGAT inhibition, DHA supplementation, or combined treatment.

### Files and variables

#### File: MDA231_DGATi_lipidomics_data_FINAL.csv

**Description:** The file shows lipidomic data from MDA-MB-231 cells treated with DGAT inhibitors, 20 μM T863 for DGAT1 and 20 μM PF-06424439 for DGAT2, and 25 μM DHA. Total cell lysates were collected at 1, 4, and 24 h following treatment.

##### Varia...,