RBM24 regulates apoptosis rates by modulating global transcriptome profile in CAL27 cells [iRIP-seq]

RNA binding proteins (RBPs) are key factors regulating post-transcriptional events. Categorized as RBPs, RBM24 expression levels have been shown to be a prognosis-related pivotal gene in oral squamous cell carcinoma. We analyzed the binding targets and regulated post-transcriptional events of RBM24 in RBM24-overexpressing cell lines and controls using iRIP-seq and RNA-seq. RBM24-overexpressing cells showed significant changes in gene expression, which are involved in biological pathways related to apoptosis and immune inflammation. RBM24-regulated genes that undergo alternative splicing are primarily engaged in biological processes related to DNA damage repair and RNA metabolism. More notably, we found that RBM24 binds to lncRNAs in addition to pre-mRNAs. These results indicated that RBM24 could play a key role in cancer progression by finding clinical therapeutic targets for Oral squamous cell carcinoma. Overall design: Cal27 cells with excessively expressed RBM24 to filtrate different expression genes and analyze for RBM24-regulated AS by RNA-seq. Next,iRlP-seq was employed to examine RBM25-binding sites.Then we used RT-qPCR to validate specific genes modulated by RBM24.