The correlation of RBCK1, RNF31 and SHARPIN protein expression levels with clinicopathological parameters.

ImageJ software was used to quantify the western blot analysis results of protein expression of interest. The protein expression levels were normalized to beta-actin as a loading control. Median was used to classify the protein expression levels into two subgroups, as low and high. Chi-square test used to show significant differences between protein expression levels and some clinicopathological parameters including ER, PR, lymph node, stage and grade statuses.