Epigenome-wide association study of human frontal cortex identifies differential methylation in Lewy body pathology. Epigenome-wide association study of human frontal cortex identifies differential methylation in Lewy body pathology

The goal of the study was to investigate if DNA methylation is associated with Lewy body pathology in human brain tissue. The Illumina Infinium MethylationEPIC Beadchip was used to obtain DNA methylation profiles across approximately 850,000 CpGs in postmortem human frontal cortex samples from European donors in the Netherlands Brain Bank. Following quality checks, sample filtering and normalization, association between CpG methylation and Braak alpha-synuclein stage was assessed using linear models. Overall design: Bisulphite converted DNA from 492 samples including 453 unique donors and 39 technical replicates were hybridised to the Illumina Infinium MethylationEPIC Beadchip v. 1.0