RNAseq_vs_DeTCT_comparison. RNAseq_vs_DeTCT_comparison

The transcriptome of zebrafish with morphologically mutant phenotypes associated with knockout alleles were analyised using Illumina sequencing to detect differential transcript abundance. Total RNA was extracted from morpholically abnormal and sibling wild typeembryos from 3 clutches using Trizol. After DNase treatment non-directional RNAseq libraries were made using the TrueSeq protocol with sample indexing. Libraries were 75 base paired-end sequenced by Illumina HiSeq. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/