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Core Mediator structure at 3.4 A extends transcription initiation complex model

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2 biological replicates of three S. cerevisiae mediator mutant strains and their parent strain were 4tU labeled. After labeled RNA purification, RNA-seq libraries were produced and sequenced.

针对三株酿酒酵母(Saccharomyces cerevisiae,简称S. cerevisiae)中介体突变菌株及其亲本菌株,各设置2个生物学重复样本并经4-硫尿嘧啶(4-thiouracil,简称4tU)标记。完成标记RNA的纯化后,构建RNA测序(RNA-seq)文库并完成测序。

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