p53-mediated DNA looping at CRMP4 promoter in colorectal cancer

An MNase-based proximity ligation assay analogous to a targeted Micro-4C approach was performed to study the DNA looping at CRMP4 promoter in colorectal cancer. Total four p53 binding sites (PBR1, PBR2, PBR3, PBR4) at CRMP4 promoter were investigated in each group. Total eleven groups (NC, PBR1 deletion by CRISPR-Cas9, PBR2 deletion, PBR3 deletion, PBR4 deletion, p53 knockdown, HIF1a knockdown, p53 TET domain deletion, p53 TET domain deletion plus wild type p53 overexpression in HCT116 cells in default, p53 knockdown in RKO cells, p53 knockdown in HCT8 cells) were enrolled in this study.