Relative quantitative real-time PCR.

Table 1 summarizes gene expression data of osteoclasts generated from BMM isolated from arthritic rats 10, 15, 20 and 40 days following immunization after 5 days of osteoclastogenic differentiation. Gene expression data of osteoclasts from arthritic rats are presented relative to gene expression of time-matched osteoclasts derived from BMM isolated from control rats. Neurotransmitter receptor genes are presented under A, osteoclast differentiation marker genes under B, genes related to cell apoptosis and survival under C and osteoclast activity marker genes under D. Data are expressed as mean ± standard error of the mean of the log fold change to calibrator (base 2). Genes of interest were normalized to GAPDH expression.
*p<0,05
**p<0,01
***p<0,001. Numbers in brackets represent the number of rats analyzed.
Relative quantitative real-time PCR.