Transcriptomic analysis of untreated log-phase Mtb H37Rv, Mtbrv0158 Knockout strain and Mtbrv0158 complemented strain

We identified rv0158 gene as a major determinant of redox homeostasis in Mtb. Disruption of rv0158 perturbed redox balance in a carbon source-specific manner, promoted killing in response to anti-TB drugs, reduced survival in macrophages, and persistence in mice. RNA sequencing analysis was performed to identify its regulon. The data indicated that rv0158 is required to balance the deployment of fatty acid substrates between lipid anabolism and oxidation. All Mtb strains were grown to early log phase (optical density =0.6) at 37 degree celsius at 180 rpm in a shaker incubator. For H2O2 (hydrogen peroxide )- treatment, Mtb strains were grown as mentioned above and then 5 mM H2O2 was added to all the strains for 2 hours, before total RNA was isolated. Two independent experiments were done for each of the strains.