The effects of novel adjuvant complex and CpG ODN on host immune response to recombinant Eimeria profilin vaccine

This study investigated the ability of two novel adjuvant formulations, QCDC (Quil A/cholesterol/DDA/Carbopol) and QCDCR (QCDC/Bay R1005), in combination with a recombinant profilin vaccine, to modulate host protective immunity and to alter new gene expression during experimental avian coccidiosis. Overall design: Four-condition experiment, Profilin only vs. Non-vaccination, Profilin only vs. Co-vaccination of QCDC plus profilin, Profilin only vs. Co-vaccination of QCDCR plus profilin, Biological replicates: 2 profilin only replicates, 2 Non-vaccination replicates, 2 QCDC plus profilin replicates, 2 QCDCR plus profilin replicates with dye-switching.

本项研究旨在探讨两种新型佐剂配方，即QCDC（Quil A/胆固醇/DDA/Carbopol）和QCDCR（QCDC/Bay R1005），与重组蛋白精氨酸疫苗联合应用，对宿主保护性免疫进行调节以及改变实验性禽类球虫病过程中的新基因表达的能力。整体设计为四条件实验，包括：仅蛋白精氨酸组、未接种疫苗组、仅蛋白精氨酸与QCDC联合接种疫苗组、仅蛋白精氨酸与QCDCR联合接种疫苗组，生物重复实验包括：2个仅蛋白精氨酸重复实验、2个未接种疫苗重复实验、2个QCDC与蛋白精氨酸联合接种疫苗重复实验、2个QCDCR与蛋白精氨酸联合接种疫苗重复实验，并采用染料切换技术。