Supporting-Detecting the combined toxicity of 17 pesticides to carboxylesterase based on fluorescence probe technology

A rapid test method for the determination of pesticide toxicity was established by using carboxylesterase (CES) and fluorescence probe <b>ACE-NH</b> based on the principle of enzyme inhibition, and this method was applied to detect the combined toxicity of 18 binary and 24 ternary pesticide combinations commonly used for fruits and vegetables to CES. The test method and results in this study can provide scientific basis for risk assessment of cumulative exposure to mixed pesticide residues