Systematic tethering approach assigns function to RNA binding proteins and identifies UBAP2L as an antagonist to FMRP-mediated translation repression (Neuron_polysome_RNA-seq)
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https://www.ncbi.nlm.nih.gov/sra/SRP154580
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In these FMRP knockout lines we then depleted UBAP2L by lentiviral delivery of two different shRNAs, with a non-targeting shRNA serving as a control. Then, differentiated all lines to cortical neurons using a directed protocol (Nehme et al., 2018), which is based on a modified version of that of (Zhang et al., 2013)). We then performed polysome profiling by measuring the ratios of transcript levels in polysome factions over inputs Overall design: RNAseq
创建时间：
2023-05-20
