Transcriptome analysis of secreted proteins responding to nutrient-selection pressure. Pochonia chlamydosporia

To determine secreted proteins that involved in adaptation of nutrient sources and response to nutrient stresses, we analyzed transcriptomes of Pochonia chlamydosporia strain 170 under three different nutrient conditions, CD (nutrient rich medium) that was predicted to repress parasitism, MM (nutrient-poor liquid minimal medium) that was predicted de-repress genes associated with parasitism, and MM-eggs(minimal medium with root-knot nematode eggs) that was prepared to induce parasitism. Overall design: Three biological replicates (a, b, c) for each of three different nutrient conditions (CD, MM, egg) were prepared and sequenced.