five

rAAV2.5T screening in HeLa-S3 cells

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA971590
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Recombinant (r)AAV2.5T was selected from the directed evolution of an AAV capsid library in human airway epithelia. The capsid gene of rAAV2.5T is a chimera of the N-terminal unique coding sequence of AAV2 VP1 (VP1u) and the VP2- and VP3-coding sequence of AAV5 with a single amino acid mutation of A581T. We conducted genome wide CRISPR screen of rAAV2.5T transduction in HeLa S3 cells using the Brunello CRISPR gRNA library.
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2023-05-11
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