Single Nucleus RNA sequencing of the mouse placental junctional zone

To characterise the transcriptome of mouse placental junctional zone cells in Control litters (deletion of the maternally-inherited and silent Igf2 allele, with normal Igf2 levels in whole litters) in UE litters (dams carried offspring with a deletion of the paternally-inherited Igf2 allele in the placental endocrine cells with diminished Igf2 production from the endocrine cells in whole litters), we performed single nucleus RNA sequencing on the placenta Junctional zone 8 Control placentas (offspring sex: 4M:4F) and 8 UE placentas (offspring sex: 4M:4F) in 2 batches each. The placental junctional zone was dissected from 8 Control and 8 UE litters and stored at -80ºC. Single nucleus suspensions were made, pooling together 2x 4 junctional zones from Control litters (offspring sex: 2M, 2F) and 2x 4 junctional zones from UE litters (offspring sex: 2M, 2F), totalling 4 different samples. Suspensions were FACS sorted for DRAQ5 positive events. Single nucleus RNA sequencing libraries were generated using the 10X Chromium single cell 3' reagents.