Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neurons

Mapping the input connections of a single neuron, or the "inputome", is crucial for constructing mesoscopic connectomes at cellular resolution of the brain. By combining retrograde viral tracing with single-cell RNA sequencing, we developed a barcoded rabies viral tracing (BRT) method that enables mapping both local and long-range input connections to transcriptome-defined neurons at the single-cell level.