Live-cell fluorescence microscopy data for: Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate

yuan_t3ss_raw_data.zip:

Microscopy data types:
- Brightfield images
- Fluorescence images (514 nm excitation)

Microscope:
Olympus IX-83 with 1.49NA oil-immersion objective (Olympus UAPON 100x)
ET442/514/561 Laser triple band set filtercube (69904, Chroma)

Camera:
Hamamatsu C9100-13 EM-CCD Camera
80 nm pixel size

yuan_t3ss_cell_objects.zip:

EPEC and C41 ColiCoords cell objects derived from the raw data. The set contains a total of 24979 individual cells (brightfield, binary, fluorescence and localization datasets for each cell) with corresponding coordinate systems.

Code to generate these cell objects from the raw data: https://github.com/Jhsmit/T3SS-paper

The data format is .hdf5 and can be read with any HDF5 reader or directly with ColiCoords: https://github.com/Jhsmit/ColiCoords

Contact:
Jochem Smit