Gene expression in MLE-12 cells transfected with microRNA (miRNA; miR) mimics upon influenza virus infection

To clarify the gene expression profile in MLE-12 cells transfected with microRNA mimics upon influenza virus infection, we transfected microRNA mimics (mmu-miR-483-3p or Negative control miRNA) into MLE-12 cells and infected A/Puerto Rico/8/1934 (PR8) strain at an MOI of 2 at 24 hours post transfection. RNA was isolated from cells at 12 hours post infection. We found that miR-483-3p transfection down-regulated the genes involved in the innate immunity regulation upon influenza virus infection. Each group had three biological replication. MLE-12 cells transfected with control miRNA mimic was used as control. After isolation of total RNA, Gene expression microarray was performed.