MicroED datasets of peptide crystals (AVAAGA) at different accumulated electron exposures

Continuous rotation data sets in SMV format were collected on a Tecnai F30 (300 kv) equipped with a TVIPS TEM CAM XF416 detector. Datasets were collected from 3 different crystals. To simulate increasing dose each crystal had 4 datasets collected from it with an approximate fluence of 3 e^-Ų per dataset (12 e^-Ųtotal accumulated exposure per crystal). Datasets at each exposure were indexed with XDS and merged with XSCALE to produce a final set of intensities at each total exposure. Experimental parameters are as follows:<br> <br> Accelerating voltage: 300 kV<br> Wavelength: 0.019687<br> Cameralength: 1320 mm<br> Exposure time: 3 s<br> Rotation speed: 0.3 degrees s^-1<br> Rotation: +/- 45 degrees <br> Detector area: 2048 x 2048 (Bin 2)<br> Pixel size: 0.031 mm<br> Spot size: 11<br> C2 aperture: 20 µm<br> SA aperture: 1 µm Datasets are grouped as follows: Crystal 1 = mov1-4, Crystal 2 = mov5-8, Crystal 3 = mov9-12. An example XDS and XSCALE file are provided. Note: It has been brought to our attention that the zip file does not always unpack in a linux environment. The following workaround was found by T. Nakane using OpenJDK (17.0.1) <code>jar -xvf</code> AVAAGA_dose_series.zip