Prdm16 Knockout Brown Adipose Tissue

Gene expression profile from brown adipose tissues of Prdm16 knockout and wile type mice. Prdm16 is a transcription factor that regulates the thermogenic gene program in brown and beige adipocytes. However, whether Prdm16 is required for the development or physiological function of brown adipose tissue (BAT) in vivo has been unclear. By analyzing mice that selectively lacked Prdm16 in the brown adipose lineage, we found that Prdm16 was dispensable for embryonic BAT development. Brown adipose tissues were collected from Prdm16 knockout and wildtype mice with 4 biological replicates per condition. Experiment was done in two separate batch for 6-week-old and 11-month-old. Extracted RNA was hybridized to Agilent two-color arrays.本数据集源自Prdm16敲除（Prdm16 knockout）与野生型（wild type）小鼠的棕色脂肪组织基因表达谱。Prdm16是一种可调控棕色与米色脂肪细胞产热基因程序的转录因子。此前，关于Prdm16是否为体内棕色脂肪组织（brown adipose tissue, BAT）的发育及生理功能所必需，尚无定论。本研究通过分析棕色脂肪谱系中选择性缺失Prdm16的小鼠，发现Prdm16并非胚胎期BAT发育所必需。研究人员从Prdm16敲除组与野生型组小鼠中采集棕色脂肪组织，每组设置4个生物学重复。实验分为两个独立批次开展，分别针对6周龄与11月龄的小鼠。提取的RNA与安捷伦（Agilent）双色基因芯片完成杂交反应。