Knockout of the Acyl CoA binding protein (ACBP) in mice - expression profile from the liver of 21 days old ACBP-/- and +/+ mice

The ACBP knockout were created by targeted disruption of the gene in mice. The expression profiling was performed on liver tissue from ACBP-/- (KO) and +/+ (WT) mice at the age of 21 days, which in our study is the time immediately before weaning. The mice used for this experiment were taken directly away from their mother. Thus, having free access to chow and breast milk until sacrificed at 8-11am 15 ACBP-/- and 15 +/+ control mice divided into 6 groups (KO1, KO2, KO3, WT1, WT2 and WT3) with 5 individuals in each group were used for this study.本研究通过靶向敲除小鼠体内的ACBP基因，构建了ACBP敲除（ACBP knockout, KO）小鼠模型。本研究对出生后21天的ACBP-/-（KO）与+/+（野生型, Wild Type, WT）小鼠的肝脏组织开展了表达谱分析；在本研究中，该时间点恰好处于断奶前夕。本实验所用小鼠均直接从母鼠处分离，因此在被实施安乐死之前，可自由采食常规饲料与母乳。本次研究共纳入15只ACBP-/-小鼠与15只野生型对照小鼠，将其分为6组（KO1、KO2、KO3、WT1、WT2、WT3），每组各5只个体，所有小鼠均于上午8时至11时被实施安乐死。