MOESM2 of Lipid metabolism of phenol-tolerant Rhodococcus opacus strains for lignin bioconversion

Additional file 2: Table S1. Excel file containing the lipid library and lipid library matches from LC/MS data. For all tabs, WTG = WT strain grown in 1 g/L glucose (glucose), 40G = evol40 grown in glucose, WTLP = WT strain grown in 0.75 g/L phenol (low phenol), 40LP = evol40 strain grown in low phenol, 40HP = evol40 strain grown in 1.5 g/L phenol (high phenol), and SD = standard deviation. Lipid species are abbreviated using their carbon number (C) and double bond number (DB), where the carbon number is the number of acyl carbons and the double bond number is the number of double bonds and cyclopropane units on acyl chains. Tab 1. TAG library. Library of triacylglycerol (TAG) species based on MSn data. Lipid species in the library that were observed in MSn analysis (Observed MSn column) are indicated using “x” (see Table 3). Tab 2. TAG data. Normalized TAG species ion counts from LC/MS data. Species amounts in each replicate (rep) are normalized by dividing the total ion counts of individual species by the total ion counts of all detected TAG species in the sample. Tab 3. PL library. Library of phospholipid (PL) species based on MSn data. Lipid species in the library that were observed in MSn analysis (Observed MSn column) are indicated using “x” (see Table 1). Tab 4. PL data. Normalized PL species ion counts from LC/MS data. Species amounts in each replicate (rep) are normalized by dividing the total ion counts of individual species by the total ion counts of all detected phosphatidylinositol (PI) or phosphatidylethanolamine (PE) species in the sample. Tab 5. MA library. Library of mycolic acid (MA) species based on MSn data. Lipid species in the library that were observed in MSn analysis (Observed MSn column) are indicated using “x” (see Table 2). Tab 6. MA data. Normalized MA species ion counts from LC/MS data. Species amounts in each replicate (rep) are normalized by dividing the total ion counts of individual species by the total ion counts of all detected MA species in the sample. Tab 7. Statistical tests. P values for statistical tests comparing C number and DB number for each lipid class between strains or growth conditions. Statistical significance was determined using a one mean, two-tailed Student’s t test with a threshold of significance of P < 0.05.