Data from: Low-coverage, whole-genome sequencing of Artocarpus camansi (Moraceae) for phylogenetic marker development and gene discovery
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https://datadryad.org/dataset/doi:10.5061/dryad.3293r
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Premise of the study: We used moderately low-coverage (17×) whole-genome
sequencing of Artocarpus camansi (Moraceae) to develop genomic resources
for Artocarpus and Moraceae. Methods and Results: A de novo assembly of
Illumina short reads (251,378,536 pairs, 2 × 100 bp) accounted for 93% of
the predicted genome size. Predicted coding regions were used in a
three-way orthology search with published genomes of Morus notabilis and
Cannabis sativa. Phylogenetic markers for Moraceae were developed from 333
inferred single-copy exons. Ninety-eight putative MADS-box genes were
identified. Analysis of all predicted coding regions resulted in
preliminary annotation of 49,089 genes. An analysis of synonymous
substitutions for pairs of orthologs (Ks analysis) in M. notabilis and A.
camansi strongly suggested a lineage-specific whole-genome duplication in
Artocarpus. Conclusions: This study substantially increases the genomic
resources available for Artocarpus and Moraceae and demonstrates the value
of low-coverage de novo assemblies for nonmodel organisms with moderately
large genomes.
提供机构:
Dryad
创建时间:
2016-06-08



