p300 and CBP are essential for skeletal muscle homeostasis, contractile function and survival

Reversible ε-amino acetylation of lysine residues regulates transcription as well as metabolic flux; however, roles for specific lysine acetyltransferases in skeletal muscle physiology and function is unknown. In this study, we investigated the role of the related acetyltransferases p300 and CBP in skeletal muscle transcriptional homeostasis and physiology in adult mice. These data reveal that p300 and CBP are required for the control and maintenance of contractile function and transcriptional homeostasis in skeletal muscle, and ultimately, organism survival. Mice with skeletal muscle-specific and inducible knockout of p300 and CBP (PCKO) were treated with tamoxifen via oral gavage for 5 days, starting at 13-15 weeks of age. At the day after the last tamoxifen gavage, mice were sacrificed after a 4 hour fast, and tissues were excised and snap-frozen. RNA was profiled in Extensor digitorum longus muscles using Agilent SurePrint G3 Mouse GE 8x60K Microarrays according to the manufacturer's instructions. There were 4 mice each in the control (wildtype) and PCKO groups.