Genotypes of case-control samples in major psychosis

We fine-mapped DNA methylation in neuronal nuclei (NeuN+) isolated by flow cytometry from post-mortem frontal cortex of the brain of individuals diagnosed with schizophrenia, bipolar disorder, and controls (n=29, 26, and 28 individuals). These genotype arrays were generated in the same samples to identify genetic-epigenetic interactions. Post-mortem brain samples were obtained through the NIH NeuroBioBank at the University of Pittsburgh; the Harvard Brain Tissue Resource Center; the Human Brain and Spinal Fluid Resource Center at Sepulveda; and the University of Miami Brain Endowment Bank. Genotypes were mapped in 103 samples (84 biological replicates: 28 control; 29 schizophrenia; 27 bipolar disorder), using genomic DNA extracted from brain tissue. 8 samples were assayed in triplicate, and the rest had no technical replicates. SNPs in each sample (n=104) were determined using the Infinium PsychArray-24 processed by The Centre for Applied Genomics (Toronto, Canada). Samples were randomized across SNP arrays. GenomeStudio was used to extract genotype data for each sample.