Bulk RNAseq analysis of dorsal root ganglia from global Nav1.7 knockout mice

We investigated changes in gene expression of dorsal root ganglia (DRG) isolated from global (pCAGG-CreER x Nav1.7 floxed) Nav1.7 knockout mice using bulk RNA sequencing. We found an upregulation of Penk and downregulation of Ceacam10 in Nav1.7 knockouts compared to controls, as well as other gene expression changes similar to those previously reported for Nav1.7 knockout mice (https://doi.org/10.1038/ncomms9967). We identified that genes selectively expressed in the C low-threshold mechanoreceptor (cLTMR) class of DRG neurons showed a general reduction in expression in Nav1.7 knockouts compared to littermate controls. Overall design: Comparative gene expression profiling by RNAseq was performed on lumbar DRGs from Nav1.7 cKO (CreER+, N = 3) and littermate control (CreER-, N = 3) mice.