five

Developmental stages, reproductive organs and other tissues

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NIAID Data Ecosystem2026-03-11 收录
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https://veupathdb.org/veupathdb/app/record/dataset/DS_ded344cb5e
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Series of RNA-Seq libraries covering larval and pupal development and adult reproductive tissues. The libraries were prepared using the Illumina Smart-Seq2 (San Diego, CA) following manufacturer protocol. Briefly, mRNAs were extracted from either individual carcasses or individual dissected tissues using RNeasy columns following standard protocols (Qiagen, Germany). Adult carcasses represent what remained after reproductive organs had been dissected. Sex was determined for individuals using morphological features for adults and pupae while L3 and L4 larvae were tested for the male-determining gene nix (Hall et al. 2016) using PCR. Aedes aegypti Liverpool strain.
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2020-04-07
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