Disruption of TFIIH activities generates a transcriptional stress gene expression response and reveals possible new targets against cancer

The global effect on transcription between cancer cells and their progenitors and investigating how these compounds affect TFIIH integrity. Here, we used an oncogenesis model to compare the effect of TFIIH inhibitors: TPL and THZ1 between transformed cells and their progenitors. RNA-seq and RNAPII-ChIP-seq experiments showed that although the levels of many transcripts were reduced, the levels of a significant number were increased after TPL treatment, with maintained or increased RNAPII promoter occupancy Overall design: RNAseq in cells MCF10A-Er-Src with TPL 4h-125 nM and RNAPII-ChIP-seq with TPL 4h-125 nM