Pseudomonas species Genome sequencing and assembly. Pseudomonas aeruginosa

Pseudomonas species, particularly Pseudomonas aeruginosa, are opportunistic pathogens of significant clinical and epidemiological importance. They are commonly associated with nosocomial infections, including sepsis, pneumonia, and urinary tract infections. Due to the intrinsic, acquired and adaptive resistance mechanisms, the pathogen developed resistance to multiple antibiotics. Whole-genome sequencing (WGS) has become unavoidable for studying molecular characteristics of pathogens including identification of potential virulence determinants, mechanisms of pathogenicity, antibiotic resistance, transmission and their molecular evolution. In addition, the degree to which antimicrobial resistant genes (ARGs) are transferred to other bacteria via mobile genetic elements (MGEs), including insertion sequences (ISs), plasmids, and phages, has a strong association with their likelihood to function as resistance transfer determinants. The findings of this study will provide valuable insights into the relationship between ARGs and MGEs in Pseudomonas species, as well as identify genetic mutations linked to antibiotic resistance in Vietnamese isolates.