TABLE 2 in Clarification of the diagnosis of the genus Loxosoma s. l. (Entoprocta; Loxosomatidae) based on morphological and molecular data

TABLE 2. Amplification primers and conditions.
MarkerPrimersPCR conditionsReference28S rRNA28SC15 min – 94°C, 35×[15 sLê et al., 1993ACC CGC TGA ATT TAA GCA T– 94°C, 30 s – 50°C, 4528SC2s – 2°C], 7 min – 72°CTGA ACT CTC TCT TCA AAG TTC TTT TC18S rRNA1F5 min – 94°C, 35×[30 sGiribet et al.,TAC CTG GTT GAT CCT GCC AGT AG– 94°C, 30 s – 49°C, 11996;5Rmin – 2°C], 7 min – 72°CWhiting et al.,CTT GGC AAA TGC TTT CGC19973FGTT CGA TTC CGG AGA GGG A18SbiGAG TCT CGT TCG TTA TCG GA18Sa2.0ATG GTT GCA AAG CTG AAA C9RGAT CCT TCC GCA GGT TCA –CT AC