Transcriptome-wide characterization of genetic perturbations

Single-cell CRISPR screens (ie Perturb-seq) of DepMap Common Essential Genes in Jurkat and HepG2 cells. Abstract: Single cell CRISPR screens such as Perturb-seq enable transcriptomic profiling of cellular perturbations at scale. However, the data produced by these screens are often noisy due to cost and technical constraints, limiting power to detect true effects with conventional differential expression analyses. Here, we introduce TRanscriptome-wide Analysis of Differential Expression (TRADE), a statistical framework which estimates the transcriptome-wide distribution of true differential expression effects from noisy gene-level measurements.