Undescribed species diversity in Brewer’s Jewelflower illuminates potential mechanisms of diversification associated with serpentine endemism

Premise of study: Documenting species-level diversity is a fundamental goal of biology, yet undescribed species remain hidden even in well-studied groups.  Inaccurate delimitation of species boundaries can limit our understanding of ecological and evolutionary processes and patterns of biodiversity, and may further impede conservation and management efforts.
Methods: We utilized an integrative approach, combining techniques from speciation biology, molecular phylogenetics, and geometric morphometrics to assess diversity in the Californian serpentine endemic Streptanthus breweri (Brewer’s Jewelflower).  We assessed reproductive isolation resulting from flowering time differences, mating system differences, and interfertility among four distinct geographic clusters of S. breweri that span the geographic range of the species.  We generated a gene tree based on the ribosomal DNA internal transcribed spacer region (ITS), a diagnostic species-level marker for this clade of jewe..., Datasets were 1) generated in greenhouse common gardens at the University of California, Davis (Davis, California, USA); and 2) using Sanger sequencing of the ribosomal DNA internal transcribed spacer region (ITS)., , # Undescribed species diversity in Brewer’s Jewelflower illuminates potential mechanisms of diversification associated with serpentine endemism

American Journal of Botany

Kyle Christie, N. Ivalú Cacho, Jacob Macdonald, Deniss J. Martinez, and Sharon Y. Strauss

Corresponding author – Kyle Christie ([[e-mail]](mailto:[e-mail]); [[e-mail]](mailto:[e-mail]))

This dataset includes:
\- six (6) .csv files,
\- two (2) .Rdata input files,
\- five (5) .R scripts used for data analysis and figure generation, and
\- one (1) .nexus file

The .R files generate Figure 1, Figure 2, Figure S2 (Appendix S2), Figure 3 and Figure 4, and Figure 5, and rely on the associated input data (.csv and .Rdata files). All missing data represented as NA.

The .nexus file (Appendix_S10_alignment_nexus) was used to generate the phylogenetic tree shown in Figure 6.

**Input data include:**

Figure1a_Sbreweri_collections_CCH2_November212023.csv
Figure1a_Sbreweri...,