Effect of obesity on gene expression of cyclooxygenase (COX)-1 and COX-2 isoforms in aortic perivascular adipose.

Adipose was collected and analyzed from animals with monogenic (GPER0, n = 5) obesity or in diet-induced obesity (DIO, n = 6), as well as from lean, age-matched WT controls (n = 6). Expression levels of mRNA were calculated based on the 2−ΔΔCT method and expressed as arbitrary units. GAPDH served as housekeeping control. *p<0.01 vs. COX-1.