Bioanalytical assay primers used for the specific detection of the sense and anti-sense strands of 372 (unmodified and modified forms). Primers were synthesised by Eurogentec (Southampton, UK). Bold A
Results of multiplexing trials of qPCR markers. Average Ct values, with the range of copy numbers in parentheses, found across 24 replicates for markers run singly or combined in a multiplex reaction.