Multigenetic phylogeny of the subfamily Larentiinae (Lepidoptera: Geometridae)

This dataset is part of a paper, which demonstrates how micro-CT can be applied to provide unambiguous illustrations of diagnostic morphological characters for new taxa description and to understand how micro-CT imaging may complement other imaging techniques. Following micro-CT scanning, a semi-automatic segmentation and volume rendering protocol was used to portray the wing venation and diagnostic structures and ornamentation of male genitalia from multiple angles. Using micro-CT images, we describe a conspicuous geometrid moth from southern Africa (Lepidoptera: Geometridae), which has been present in collections since 1894 but left without an available name. Using a multigenetic dataset comprising 273 terminal taxa from the superfamily Geometroidea, we constructed a molecular phylogeny to place our study species to an isolated lineage in Geometridae: Larentiinae, tribe Xanthorhoini sensu lato. We describe it as Chloecolora vergetaria new genus, new species Englund & Staude, and p..., One entire mid-leg or femur, when no complete leg was available, was detached from the Chloecolora vergetaria sp. n. specimens for DNA extraction. Genomic DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit following the manufacturer’s protocol at the Finnish Museum of Natural History (FMNH) molecular laboratory. DNA amplification and sequencing were carried out following protocols proposed by Wahlberg and Wheat (2008): one mitochondrial gene (mtCOI) and six protein-coding nuclear gene regions, Arginine Kinase (ArgK), sarco/endoplasmic reticulum calcium ATPase (Ca-ATPase), Elongation Factor 1 alpha (EF-1alpha), sorting Nexin-9-like (Nex9), Ribosomal Protein (RpS5), and wingless (wgl). 
The molecular data extracted from three specimens were included in the dataset of a total of 273 terminal taxa. The remaining 272 terminal taxa, with up to 11 genes per species, were obtained from published data by Murillo-Ramos (Murillo-Ramos et al., 2019). All subfamilies of the family Geomet..., , # Larentiinae\_Full\_Tree\_FigS4; Multigenetic phylogeny of the subfamily Larentiinae (Lepidoptera: Geometridae)

[https://doi.org/10.5061/dryad.kd51c5bd1](https://doi.org/10.5061/dryad.kd51c5bd1)

The data file contains fasta and tree files used to construct a molecular phylogeny of the subfamily Larentiinae (Lepidoptera: Geometridae). It is part of the manuscript \"130 years from discovery to description: micro-CT scanning applied to construct the integrative taxonomy of a forgotten moth from Southern Africa (Lepidoptera: Geometridae)\" that was submitted for referral in a scientific journal as of 2.11.2023.

## Description of the data and file structure

The tree consists of 275 protein-coding nuclear and mitochondrial sequences from 273 terminal taxa from the superfamily Geometroidae (Lepidoptera); 2 of them from Uranidae forming an outgroup and the rest from Geometridae, 195 from the subfamily Larentiinae.

## Sharing/Access information

The tree contains novel sequences from three s...