Quantitive analysis of transcriptome alteration after treatment with J007(a hnrnp A1 inhibitor) in murine myeloma cells

Purpose: To perform an unbiased evaluation of altered gene expression in J007 treated MM cells. Methods:mRNA profiles in myc-driven murine myeloma cells both before and after J007 treatment were generated by Illumina HiSeq 3000 sequencer. The differential gene expressions were examined using DESeq2 algorithm. QIAGEN Ingenuity Pathway Analysis (IPA) software was used for pathway enrichment analysis of differentially expressed genes. Results: 257 genes that were upregulated greater than 2x fold and 230 genes that were downregulated at least 50% by J007. The most significantly altered gene set was that of ‘myc targets. The ‘angiogenesis’, ‘mTORC1 signaling’, ‘protein secretion’, ‘G2M checkpoint’, ‘unfolded protein response’ and ‘fatty acid metabolism’ gene sets were also decreased by J007 . Conclusion: The results support that J007 functions as expected to inhibit hnrnpa1 and c-myc binding in vivo. Comparison of mRNA profiles before and after J007 treatment